User:Easilva/Notebook/Gels with PGE2/2012/07/26

Gels with PGE2 - Collaboration

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Entry Quench oxidation with 6 ul of Ethylene Glycol. 30 min at room temperature with fast stirring; transfer solutions for dialysis membranes; start dialysis (4L of dd H2O; changed 3x first 24 hrs, twice next 3 days); soak the tubes for ~ 30 min before adding the solutions; water changed at 19h25; Notes Both (LF10/60 and LF 20/40) displayed a slighted yellow coloration that became visible when adding sodium periodate - see attached picture Dialysis tubing details 45 mm (flat width); 29 mm (diameter); vol./length= 6.4ml/cm; MWCO: 3500 - Vendor VWR#S632724