User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/08/19
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High stability plasmid developmentAbstract
LigationUsing the electrophoresis gel performed by Fibo we determined the concentration of both the B0015 part and the pSB1T3 backbone both digested with EcoRI and PstI. The B0015 part is ten times as concentrated as the backbone, aproximately. We designed the following reactions varying the volume of the DNA solutions:
Oligonucleotide DilutionsThe following oligonucleotides were diluted using the concentration formula to 500μL at 5μM: C0V0 = C1V1
repC PCRI prepared a 50μL PCR with the Platinum Taq DNA polymerase with the following concentrations:
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