User:Eduardo Vladimir Munoz/Notebook/UNAM Genomics Mexico 2011/2011/09/01

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High Stability Plasmid Development Abstract Double digestions of B0014, B1007, P1004 Double Digtestions DD The following three DD were performed to fill a total volume of 100μL each: B0014 Bidirectional Terminator B0014 in pSB1AK3 86μL BSA 100x 1μL Buffer 4 10μL EcoRI-HF 1.5μL XbaI 1.5μL Total 100μL B1007 Bidirectional Terminator B1007 in pSB1AK3 86μL BSA 100x 1μL Buffer 4 10μL EcoRI-HF 1.5μL XbaI 1.5μL Total 100μL P1004 Chloramphenicol Resistance P1004 in pSB1A1 86μL BSA 100x 1μL Buffer 2 10μL EcoRI-HF 1.5μL SpeI 1.5μL Total 100μL