Plasmid extraction of the B0015 dephosphated and repC ligation
Gels to corroborate the identity of strains 2, 10 and 11
Extractions
Two strains (labeled 3 and 5) were inoculated into liquid LB media and grown for plasmid extraction.
Electrophoresis gels
The digestions gels had not enough concentration to corroborate the positives. Yet, the PCR amplification revealed that the repC region is only contained in strains 2 and 10.