Biomod/2012/TeamSendaiA/Methods

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Team Sendai > Team Sendai A Top> Methods

AFM

AFM

  AFM enables us to observe nano size structures composed of DNA. Make an observation sample. We put an observation object on a mica for five minutes and wash it in buffer. (Tris/Tris-HCL 20 mM Mg2+ 12.5 mM pH = 7.4)

Electrophoresis

AFM   We can see whether constructing DNA structures is achieved or not by Electrophoresis. It also informs us of DNA length of approximately.
  The electrophoretic condition is cf. Result & Method.

How to create micelle

soni   We dissolve lipid (OcTMAB) in chloroform. Take a small amount of solution, blow-dry it completely with a stream of argon gas. Add TAE Mg2+ buffer or mQ and multiply the high frequency by ultrasonic liquid processor.

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