User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/21
|Gel Electrophoresis of PCR Product|| Main project page|
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To determine whether or not the PCR performed on 09/20/11 of the DNA coding for a mutant GFP was successful using agarose gel electrophoresis.
A photo of the electrophoresis gel is displayed below:
The first column is the ladder. The next columns are groups 1, 2, 3, 4, and 5.
Gel ladder: 500 bp, 1000 bp, 2000 bp, 3000 bp, 4000 bp, 5000 bp, 6000 bp, 7000 bp, 8000 bp, 9000 bp, 10000 bp
An optimum gel would have used 1.5% agarose.