User:Elaine Marie Robbins/Notebook/CHEM-496/2011/10/18

From OpenWetWare

Jump to: navigation, search
Conductivity of AuNP III, Fluorophore Main project page
Previous entry      Next entry


Conductivity of AuNP III

To run the conductive plates made on 10/12/11 to determine the conductivity of AuNP.


To make a labeled cysteine fluorophore.



  1. Combine 2 mg of 5-iodoacetamido fluorescein (5-IAF) with excess cysteine (0.39 mg) in 3.95 mL of 50 mM Tris buffer (pH 7.5).
  2. Vortex to mix.
  3. Wrap reaction tube in foil and allow reaction to proceed for 2 hr.
  4. Add 100 μL of dithiothreitol (DTT)


Conductivity of AuNP II

The Pd plates broke off of the Teflon plate, so the test was not conducted.


Fluorophore and references used in this experiment:

Personal tools