User:Elaine Marie Robbins/Notebook/CHEM-496/2011/11/30

Objective

To try to control the aggregation process of AuNP/BSA.

Description

1. Make 4 solutions of AuNP:
   • Solutions 1 and 2 (Au/BSA mole ratio = 70)
     1. Combine 1 mL of 2.9 mM HAuCl₄ and 2.41 mL of 0.0172 mM BSA in a test tube.
     2. Dilute to 9 mL with water.
     3. Place in oven at 70°C.
   • Solutions 3 and 4 (Au/BSA mole ratio = 166)
     1. Combine 1 mL of 2.9 mM HAuCl₄ and 1.02 mL of 0.0172 mM BSA in a test tube.
     2. Dilute to 9 mL with water.
     3. Place in oven at 70°C.
2. Leave solutions 1 and 3 in oven for 3.5 hr.
3. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.

Data

Solution 1: Au/BSA = 70, left in the oven

Solution 2: Au/BSA = 70, taken out of the oven every 30 minutes

Solution 3: Au/BSA = 166, left in the oven

Solution 4: Au/BSA = 166, taken out of the oven every 30 minutes

Notes

Purple fibers began to form in solutions 3 and 4 after 1 hr. Purple solution began to form in solution 1 after 1.5 hr.