User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/14

Objective

To continue the experiment performed on 02/01/12 to determine the effects of various concentrations of tris buffer on AuNP absorbance.

Description

1. Take UV-vis data for various dilutions of tris buffer (pH 10.9)
   • 1 M
   • 500 mM
   • 200 mM
   • 100 mM
   • 75 mM

Data

• Graph of absorbance vs wavelength for AuNP and different dilutions of tris

While all concentrations of tris buffer cause a peak shift towards shorter wavelengths, the higher concentrations tended to yield lower absorbance values.

• Graph of peak absorbance vs -log(ionic strength of tris buffer solution).

• Graph of peak wavelength vs -log(ionic strength of tris buffer solution).

Notes