User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21
|Effect of Tris Buffer on Protein Fibers II|| Main project page|
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To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12, and to synthesize protein fibers.
High absorbance values obtained from sample prior to centrifuging is most likely due to light scattering caused by solid protein fibers.
The presence of a peak at 500-550 nm after centrifuging indicates that AuNP may be present in solution, even though the solution is a 166 Au/BSA ratio solution. Prior to the addition of the 50 mM tris solution (pH 10.1), all Au particles were associated with the protein fibers, and none were in solution.