User:Elaine Marie Robbins/Notebook/CHEM-496/2012/02/21

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Effect of Tris Buffer on Protein Fibers II Main project page
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To take UV-vis data of tris + 50 mM tris buffer (pH 10.1) mixed on 02/15/12, and to synthesize protein fibers.


Synthesizing AuNP

  1. Combine 1 mL of 3.66 mM HAuCl4 and 1.27 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 10 mL with water.
  3. Place in oven at 75°C.
  4. Leave solutions in oven for 3 hr.

Determining the effect of tris buffer on 166 Au/BSA ratio solution

  1. Take UV-vis data of 166 Au/BSA ratio solution synthesized on 01/31/12 and modified on 02/15/12.
  2. Centrifuge for 10 min at 13,200 rpm.
  3. Take UV-vis data of supernatant.


  • Graph of absorbance vs wavelength for UV-vis data obtained before and after sample was centrifuged, compared with data obtained on 02/15/12.
Image:Absorbance vs wavelength 2-21-12.jpg

High absorbance values obtained from sample prior to centrifuging is most likely due to light scattering caused by solid protein fibers.

  • Magnified graph of absorbance vs wavelength not including data obtained before centrifuging sample.
Image:Absorbance vs wavelength magnified 2-21-12.jpg


The presence of a peak at 500-550 nm after centrifuging indicates that AuNP may be present in solution, even though the solution is a 166 Au/BSA ratio solution. Prior to the addition of the 50 mM tris solution (pH 10.1), all Au particles were associated with the protein fibers, and none were in solution.

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