User:Elaine Marie Robbins/Notebook/Capstone/2012/04/05

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Data Analysis, Elongation II Main project page
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Methods

  1. Repeat elongation procedure from 03/28/12:
    1. Mix 3 μL AuNP/TS primer, 0.5 μL 50x dNTP mix, 16 μL sterile water, 2.5 μL 10x PCR buffer, and 2 μL cell extract in a PCR tube.
    2. Incubate in an oven at 30°C for 30 min.
    3. Store in refrigerator.
  2. Take UV-vis data of samples:
  3. Take emission fluorescence data of samples (excited at 515 nm):

References

Yi Xiao, Karen Y. Dane, Takanori Uzawa, Andrew Csordas, Jiangrong Qian, H. Tom Soh, Patrick S. Daugherty, Eric T. Lagally, Alan J. Heeger, and Kevin W. Plaxco. Detection of Telomerase Activity in High Concentration of Cell Lysates Using Primer-Modified Gold Nanoparticles. Journal of the American Chemical Society 2010, 132, 15299-15307.



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