User:Elaine Marie Robbins/Notebook/Capstone/2012/06/14

From OpenWetWare

Jump to: navigation, search
Fluorescence of MB Open and Closed Main project page
Previous entry      Next entry

Methods

  1. Take fluorescence data of the MB, the complementary strand, and both together.
  2. Make solutions containing either citrate-capped or BSA-conjugated AuNP and various cell dilutions:
    1. Mix 3 μL AuNP/TS primer, 1 μL 50x dNTP mix, 16 μL sterile water, 2.5 μL 10x PCR buffer, and 2 μL cell extract in a PCR tube.
      • Cell dilutions used: 400000, 200000, 100000, 50000, 25000.
    2. Incubate in heat block at 30°C for 30 min.
    3. Add 6.10 μL molecular beacon.
    4. Incubate in heat block at 37°C for 1 hr.
    5. Take fluorescence spectra.

Data

  • Fluorescence of MB open and closed, excited at 515 nm, 10 nm slit width:
Image:12-06-14 fluorescence of MB open and closed.png


  • Fluorescence of solutions containing citrate-capped AuNP, excited at 515 nm:
Image:12-06-14 fluorescence of citrate.png
Image:12-06-14 fluorescence of citrate magnified.png


  • Peak fluorescence intensity vs. number of cells for citrate-capped AuNP:
Image:12-06-14 intensity vs number of cells citrate.png


  • Fluorescence of solutions containing BSA-conjugated AuNP, excited at 515 nm:
Image:12-06-14 fluorescence of BSA.png
Image:12-06-14 fluorescence of BSA magnified.png


Personal tools