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Elaine Marie Robbins/Notebook/Capstone/2012/07/13
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Capstone
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2012
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FCS of Open MB
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Methods
Make solutions of 200 nM MB open and closed.
Open MB solution contains 10x excess of complimentary strand.
Incubate solutions at 75°C for 30 min.
Incubate solutions at room temp for 30 min.
Run fluorescence.
Run FCS.
Data
Fluorescence of 200 nM MB with 10x excess primers, scan speed 10 nm/min:
Fluorescence of MB with fluorescein comparison:
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