User:Eleni N. Kalivas/Notebook/CHEM-571/2013/11/06

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Objective

Our objective for today was to run UV-vis on the Lysozyme-AuNPs and Myoglobin-AuNPs samples that were synthesized last week. Today we will also be synthesizing new lysozyme-AuNPs and also new Hemoglobin-AuNPs solutions

Protocol

  • The samples that were being run on the UV-vis were first centrifuged for 30 minutes before each spectra was taken.
  • Protein:AuNP solutions, details of how the solutions were prepared can be found here.
  1. Lysozyme-AuNPs (protein:Au ratio):
    1. 5:1
    2. 10:1
    3. 15:1
    4. 20:1
    5. 25:1
    6. 30:1
    7. 35:1
    8. 40:1
    9. 45:1
    10. 50:1
  2. Hemoglobin-AuNPs (protein:Au ratio)
    1. 30:1
    2. 50:1
    3. 70:1
    4. 90:1
    5. 110:1
    6. 130:1
    7. 150:1
    8. 170:1
    9. 190:1
    10. 210:1

Data

Absorbance Spectra of Lysozyme-AuNPs

Absorbance of Myoglobin-AuNPs

Additional Observations

  • Lysozyome-AuNPs: Only the 30:1 ratio exhibited an absorbance peak at 526nm, this solution appeared colored and did not form fibers. The peak and color observations are characteristic of no fiber formation.
  • Myoglobin-AuNPs: Only the 15:1 ratio exhibited an absorbance peak at 732nm, this peak represents the optimal concentration for the formation of fibers.



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