User:Eleni N. Kalivas/Notebook/CHEM-571/2014/02/04

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Objective

Characterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis.

Protocol

  1. Remove 1 mL aliquot from test tube and place in the cuvette.
  2. Take the UV-Vis spectra of the sample.
  3. Take the fluorescence spectra of each sample
  4. Repeat for each test tube
  5. Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate.
  6. Data analysis of the UV-Vis absorbances, and fluorescence spectra
  1. Determine if any changes in procedure need to be made.
    1. Is a different scale of protein:ligand ratio required?
    2. Were QD NCs successfully synthesized?
  2. Prepare stock solutions for next synthesis on the following day.

Note: Much of the protocol for today will be determined by the results of our first synthesis.

Edited Protocol

  • we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three.


Corrected Volumes Table

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 40 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM L-Cysteine (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
054060600060030006005.4
10540606006054030006005.4
205406060012048030006005.4
305406060018042030006005.4
405406060024036030006005.4
505406060030030030006005.4
605406060036024030006005.4
705406060042018030006005.4
805406060048012030006005.4
90540606005406030006005.4
10054060600600030006005.4

Notes

Add before and after photos, and get uv-light photo.
UV-Vis of BSA-L-Cysteine




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