User:Eleni N. Kalivas/Notebook/CHEM-571/2015/01/28

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Objective First trial of synthesizing PVA-Alginate beads with and without NaMT Protocol Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992) Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) ~0.1 g of nanocomposites should be added to this mixture where applicable Cool dissolved and mixed solution to 35°C Prepare 210 mL of saturated citric acid with 2% calcium chloride Add 4.2 grams of calcium chloride to 210 mL of water Add citric acid to saturation Add the PVA-Alg-Nanocomposite mixture drop-wise to the gently stirring citric acid-calcium chloride solution using a thin needle Stir for 24hrs to allow solidification Rinse with water to remove excess reactants Measurements PVA-Alg 1.015 g PVA 0.1649 g alginate PVA-Alg #2 0.998 g PVA 0.159 g Alg PVA-Alg-NaMT 0.999 g PVA 0.161 g alginate 0.109 g NaMT Saturated citric acid, 2% calcium chloride solution 4.224 g (PVA-Alg), 4.231 g (PVA-Alg-NaMT), 4.207 g (PVA-Alg #2) calcium chloride 32.363 g (PVA-Alg), 32.330 g (PVA-Alg-NaMT), 32.267 g (PVA-Alg #2) citric acid Notes PVA-Alg beads pumped with syringe made fiber like clusters not cooled at all dropped in the middle PVA-Alg-NaMT beads made with disposable dropper cooled 20 seconds in dropper dropped along side Turn heat off but keep stirring to prevent clumping pre-bead forming Let cool in dropper before dropping Move to Dr Fox's automated pumper when repeating bead synthesis Use wide dish PVA-Alg #2 done in wide dish, dropped with disposable dropper, turn off heat ~1 min before dropping Don't fill disposable dropper all the way > cooling solution will come out