User:Eleni N. Kalivas/Notebook/CHEM-571/2015/01/30

PVA-Alg Bead Synthesis

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January 30, 2015 Malachite Green calibration curve redo PVA-Alg/PVA-Alg-NaMT bead synthesis, same scale, 4% CaCl, smaller beads using glass pipette Procedure Procedure adapted from | Kuo-Ying Amanda Wu and Keith D. Wisecarver (1992)<br.> Modified from 1/28/2015 Prepare an aqueous solution of 13% PVA and 0.02% sodium alginate at 60°C (Stir plate on 3rd setting: ~68°C) 1 gram of PVA and 0.16 g of sodium alginate in 7 mL of water (12.5% PVA and ~0.02% alginate) Add alginate while stirring PVA-water solution ~0.1 g of nanocomposites should be added to this mixture where applicable Cool dissolved and mixed solution to 35°C Prepare 210 mL of saturated citric acid with 2% calcium chloride Add 8.4 grams (4%) of calcium chloride to 210 mL of water Add citric acid to saturation (32 g citric acid) 14.7g/100mL > 30.87 g/210 for solubility comparable to boric acid Add the PVA-Alg-Nanocomposite mixture drop-wise (by syringe) to the gently stirring (stir plate 3) citric acid-calcium chloride solution using a Pasteur pipette Stir for 2 hrs to allow solidification Rinse with water to remove excess reactants Measurements PVA-Alg PVA: 1.044 g Alginate: 0.16 g CaCl: 8.213 g citric acid: 32.01 g PVA-Alg-NaMT PVA: 0.994 g Alg: 0.156 g NaMT: 0.103 g CaCl: 8.381 g citric acid: 33.01 g PVA-Alg-NaMT #2 PVA: 1.098 g Alginate: 0.159 g NaMT: 0.125 g CaCl: 8.9 g citric acid: 32.02 g CaCl: 8.403 g citric acid: 32.04 g Vial, no cap: 13.023 g Notes PVA-Alg does not work PVA-Alg-NaMT #2 made in sonicator Next Week Madeleine: MG Calibration curve Eleni: Scale up PVA-Alg-NaMT synthesis James: start next nanocomposite synthesis