User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/09/07

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Objective

To synthesize gold nanoparticles (AuNPs) from bovine serum albumen (BSA) and HAuCl4

Description

This experiment uses the same procedure that was used on August 31, 2011.

Changes to the Experiment

Two reactions were run in this experiment. One reaction took place in the same buffer solution used on August 31. The other reaction took place in water. The final concentration of the solution was 0.25 mM of HAuCl4 and 1.5 uM of BSA in 10 mL of H2O or buffer. To make these solutions, 1 mL of 25 mM chloroauric acid and 1 mL of 15 uM BSA in H2O were placed in each reaction.


Data

Image:Wavelength_vs._Absorbance_Water_Sept_7.png


Image:Time_vs._Absorbance_at_550_Water_Sept_7.png


Image:Wavelength_vs._Absorbance_Buffer_Solution_Sept._7.png‎


Image:Time_vs._Absorbance_at_550_Buffer_Solution_Sept_7.png‎

Notes

  • Slightly less than 1 mL of 15 uM BSA in H2O was added to the reaction tube containing the buffer solution with a pH of 4.3
  • At the beginning of the reaction, the solution had a clear color. At the end of the first 30 minutes of the reaction, the test tube containing the buffer was cloudy and had particles floating around, while the solution in water was still clear. By the 60 minute point of the reaction, the test tube containing the water had floating golden strands with clear balls. 90 minutes into the reaction, the golden strands became purple and had a more clump like shape.



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