User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2012/02/14

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Objective

Three reactions with the fluorescent dye were carried out.

Description

  1. 50 mM Tris buffer (pH 8.73) was added to 10 mL solutions of gold nanoparticles, purple fibers, and a BSA control containing BSA and HCl until the pH of the solutions was 8.50.
  2. 2 mg of the dye was measured out and dissolved in 160 μL DMSO. This was repeated two additional times.
  3. The dye was added to the reaction mixtures with a pH of 8.50.
  4. The reaction tubes were covered in foil and allowed to shake for 24 hours at room temperature.

Data

  • When raising the pH of the BSA control, 326 μL of the Tris buffer was needed. (Initial pH = 3.98, final pH = 8.50)
  • When raising the pH of the purple fibers, 1.5 mL of the Tris buffer was used. (Initial pH = 3.17, final pH = 8.51)
  • When raising the pH of the purple solution, 550 μL of the Tris buffer was used. (Initial pH = 3.50, final pH = 8.50)

Notes

  • When the dyes were added to the solutions, color changes were observed.
  • When the dye was added to the purple solution, the color changed from a light purple/pink color to dark purple on the top and fuchsia at the bottom.
  • When the dye was added to the purple fibers, the solution turned blue.
  • When the dye was added to the control, the reaction turned blue on top and purple on the bottom.



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