User:Eric M. Walters/Notebook/Spring 2012/2012/05/30

From OpenWetWare

Jump to: navigation, search
Cyanobacterial acrylate production Main project page
Previous entry      Next entry

Colony PCR from flanking primers

Image:30 colony PCR 1.jpg

This is a gel of the PCR run yesterday. This could be false negatives, since if there were only one copy of the barcode sequence inserted, it would likely not show up. So today I'm repeating it with the BC-F primer at 52°C.

10 uL GoTaq reactions

  • 5uL GoTaq mastermix
  • .5 uL BC-F
  • .5 uL DnRv1
  • 4 uL water

Colony PCR

Image:Pflegerlab 2012-05-30 17hr 10min internal colony PCR.jpg


Personal tools