User:Fermenter User/Notebook/BROM C4

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM C4 (F7)


Day 0 (Jun 1 Mon):

Change #1: Media composition: JR
   <Natasha>             6/1/2009 (10:30) Bring media to fermenter room
<Sung-Hye> 6/1/2009 (11:00) Assemble bioreactor 6/1/2009 (11:00) Add 10 ml of Antifoam 6/1/2009 (11:10) Start Sterilization (Liq 45 min) 6/1/2009 (12:50) Cooling of bioreactor (Temp setup) 1 L/min air, 400 rpm 6/1/2009 (12:55) Start Fermentation Software for Fermenter #7 6/1/2009 (13:05) Connect Acid (250 ml) and Base (340 ml after prime pump and bring pH to 7.0) Start pH control

Day 1 (Jun 2 Tue):

                         6/2/2009 (09:00) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min
                         6/2/2009 (09:00) Set dO2
                         6/2/2009 (09:00) Completion of bioreactor set up: Ready for inoculation


   <Natasha>             6/2/2009 (11:15) Inoculation: inoculation volume (300 ml) Fermentation 0:00

<Natasha> 6/2/2009 (11:20) Sampling Fermentation 0:05 Cell weight (10 mg), Activity (65%, x100), Activity (25% , x1000) <Natasha> 6/2/2009 (17:55) Sampling Fermentation 6:35 Cell weight (77 mg), Activity (92%, x100), Activity (65%, x1000)

Day 2 (Jun 3 Wed):

   <Sung-Hye>            6/3/2009 (8:15) Fermentation 09:00
                                           Replace O2 tanks
   <Natasha>             6/3/2009 (9:30) Sampling  Fermentation 22:05
                                           Cell weight ( 156 mg), Activity (  90%, x100), Activity (   77 %, x1000)
   <Vincent/Natasha>     6/3/2009 (10:45) Fermentation 11:30
                                           Harvest Fermentation






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