User:Fermenter User/Notebook/BROM C6

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM C6


Day 0 (Mar 29 Mon):

   <Natasha>             3/29/2010 (10:00) Bring media to fermenter room
<Sung-Hye> 3/29/2010 (11:00) Assemble bioreactor 3/29/2010 (11:00) Add 5 ml of Antifoam/fermenter 3/29/2010 (12:10) Start Sterilization (Liq 45 min) 3/29/2010 (13:55) Cooling of bioreactor (Temp setup) 1 L/min air, 300 rpm 3/29/2010 (13:58) Start Fermentation Software for Fermenter #6 3/29/2010 (13:59) Start Fermentation Software for Fermenter #7 3/29/2010 (14:30) Connect Acid (250 ml) and Base (400 ml) Start pH control 3/29/2010 (14:30) Calibrate dO2 probe with 300 rpm, Air purge for 30 min 3/29/2010 (14:30) Set dO2


   <Natasha>             3/29/2010 (15:00) Inoculation: inoculation volume (300 ml) Fermentation 0:00
                                           F6: Cell weight (?? mg), Activity (26%, x100), Activity (11% , x1000)
                                           F7: Cell weight (?? mg), Activity (37%, x100), Activity (23% , x1000)


Day 1 (Mar 30 Tue):

   <Natasha>             3/30/2010 (10:00) Sampling  Fermentation 16:00
                                           F6: Cell weight (?? mg), Activity (93%, x100), Activity (75% , x1000)
                                           F7: Cell weight (?? mg), Activity (86%, x100), Activity (45% , x1000)


   <Natasha>             3/30/2010 (13:30) Sampling  Fermentation 22:30
                                           F6: Cell weight (?? mg), Activity (93%, x100), Activity (75% , x1000)
                                           F7: Cell weight (?? mg), Activity (96%, x100), Activity (88% , x1000)
   <Sung-Hye&Natasha>    3/30/2010 (14:00) Fermentation 23:00
                                           Harvest Fermentation
                                           F6: Acid (200 ml), Base (200 ml)
                                           F7: Acid (250 ml), Base (280 ml)






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