User:Fermenter User/Notebook/BROM C7

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM C7


Day 0 (Apr 20 Tue):

   <Natasha>             4/20/2010 (11:30) Bring media to fermenter room
<Sung-Hye> 4/20/2010 (13:00) Assemble bioreactor 4/20/2010 (13:00) Add 5 ml of Antifoam/fermenter 4/20/2010 (13:50) Start Sterilization (Liq 45 min) 4/20/2010 (15:30) Cooling of bioreactor (Temp setup) 1 L/min air, 300 rpm 4/20/2010 (15:44) Start Fermentation Software for Fermenter #6 4/20/2010 (15:46) Start Fermentation Software for Fermenter #7 4/20/2010 (16:00) Connect Acid (250 ml) and Base (400 ml) Start pH control 4/20/2010 (16:10) Calibrate dO2 probe with 300 rpm, Air purge for 30 min 4/20/2010 (16:10) Set dO2


   <Natasha>             4/20/2010 (16:30) Inoculation: inoculation volume (300 ml) Fermentation 0:00
                                           F6: Cell weight (?? mg), Activity (23%, x100), Activity (9% , x1000)
                                           F7: Cell weight (?? mg), Activity (37%, x100), Activity (23% , x1000)

Day 1 (Apr 21 Wed):

   <Sung-Hye>            4/21/2010 (9:30)  Increase rpm to 500 Fermentation 16:00
                                           F6: Acid (250 ml) Base (230 ml)
                                           F7: Acid (210 ml) Base (230 ml)
   <Natasha>             4/21/2010 (11:00) Sampling  Fermentation 17:30
                                           F6: Cell weight (?? mg), Activity (97%, x100), Activity (74% , x1000)
                                           F7: Cell weight (?? mg), Activity (97%, x100), Activity (79% , x1000)


   <Natasha>             4/21/2010 (14:30) Sampling  Fermentation 22:00
                                           F6: Cell weight (?? mg), Activity (96%, x100), Activity (74% , x1000)
                                           F7: Cell weight (?? mg), Activity (95%, x100), Activity (82% , x1000)
   <Sung-Hye&Natasha>    4/21/2010 (15:00) Fermentation 22:00
                                           Harvest Fermentation
                                           F6: Acid (250 ml), Base (180 ml)
                                           F7: Acid (210 ml), Base (140 ml)






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