User:Fermenter User/Notebook/BROM C8

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM C8


Day 0 (Aug 23 Mon):

   <Natasha&Henry>       8/23/2010 (12:00) Bring media to fermenter room
<Sung-Hye> 8/23/2010 (12:00) Assemble bioreactor 8/23/2010 (12:00) Add 10 ml of Antifoam/fermenter 8/23/2010 (13:55) Autoclave (Liq 45 min) 8/23/2010 (15:30) Cooling of bioreactor (Temp setup) 1 L/min air, 300 rpm 8/23/2010 (15:46) Start Fermentation Software for Fermenter #6 8/23/2010 (15:48) Start Fermentation Software for Fermenter #7 8/23/2010 (16:00) Connect Acid (250 ml) and Base (400 ml) Start pH control 8/23/2010 (16:40) Calibrate dO2 probe with 300 rpm, Air purge for 30 min 8/23/2010 (16:40) Set dO2


   <Natasha&Henry>       8/23/2010 (17:00) Inoculation: inoculation volume (300 ml) Fermentation 0:00
                                           F6: Cell weight (?? mg), Activity (15.7%, x100), Activity (42.1% , x1000)
                                           F7: Cell weight (?? mg), Activity (15.3%, x100), Activity (37.9% , x1000)

Day 1 (Aug 24 Tue):

   <Sung-Hye>            8/24/2010 (9:00) Increase rpm to 550
   <Natasha&Henry>       8/24/2010 (10:00) Sampling  Fermentation 19:00
                                           F6: Cell weight (?? mg), Activity (1.56%, x100), Activity (15.4%, x1000)
                                           F7: Cell weight (?? mg), Activity (0.99%, x100), Activity (2.49%, x1000)
   <Sung-Hye>            8/24/2010 (11:30) Fermentation 21:00
                                           Harvest Fermentation
                                           F6: Acid (220 ml), Base (200 ml)
                                           F7: Acid (210 ml), Base (250 ml)






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