User:Fermenter User/Notebook/BROM C9

From OpenWetWare

Jump to: navigation, search
Customize your entry pages


Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

THIS IS BROM C10 (F4)


Day -1 (Dec 20 Mon):

   <Henry>              12/20/2010 (15:00) Bring concentrated 4-L media to fermenter room
(first time prepared, diluted too much. second time prepared again) <Sung-Hye> 12/20/2010 (14:30) Start Fermentation Software for Fermenter #4 12/20/2010 (15:00) Dilute with water to 40-L final 12/20/2010 (15:00) Add 40-ml of Antifoam (10-ml Antifoam/10-L) 12/20/2010 (15:30) Start Sterilization 12/20/2010 (16:00) Complete Sterilization 12/20/2010 (16:10) Cooling of bioreactor (Temp setup) 10 L/min air, 50 rpm

Day 0 (Dec 21 Tue):

                        12/21/2010 (11:20) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min
                        12/21/2010 (11:20) Set dO2
                        12/21/2010 (11:20) No Acid and Base (1000 ml)
                        12/21/2010 (11:20) Start pH control                   
   <Henry&Sung-Hye>     12/21/2010 (13:00) Inoculation: inoculation volume (1800 ml) Fermentation 0:00

<Henry> 12/21/2010 (13:00) Sampling Fermentation 0:00 Cell weight (??? mg), Activity ( 95.2%, x10), Activity ( 67.3%, x100)

Day 1 (Dec 22 Wed):

   <Sung-Hye>            12/22/2010 (10:30) Fermentation 21:30
                                           Refill Base (+1000 ml)
   <Henry>              12/21/2010 (10:30) Sampling  Fermentation 21:30
                                         Cell weight (??? mg), Activity ( 86.7%, x100), Activity ( 49.4%, x1000)
   <Henry>              12/21/2010 (11:30) Sampling  Fermentation 22:30
                                         Cell weight (??? mg), Activity ( 86.2%, x100), Activity ( 52.0%, x1000)
   <Henry>              12/21/2010 (13:30) Sampling  Fermentation 24:30
                                         Cell weight (??? mg), Activity ( 83.3%, x100), Activity ( 39.6%, x1000)
   <Henry>              12/21/2010 (15:00) Sampling  Fermentation 26:00
                                         Cell weight (??? mg), Activity ( 84.6% x100), Activity ( 40.6%, x1000)
   <Sung-Hye$Henry>     12/21/2010 (15:40) Harvest




Recent changes



Personal tools