User:Fermenter User/Notebook/BROM E2

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

BROM E2


Day 0 (Mar 16 Mon):

   <Jadwiga>             3/16/2009 12:15 Bring media to fermenter room
   <Sung-Hye>            3/16/2009 12:15 Assemble bioreactor 
                         3/16/2009 12:20 Add 5 ml of Antifoam
                         3/16/2009 12:20 Adjust pH to 7.0
   <Sung-Hye>            3/16/2009 12:30 Start Sterilization (Liq 45 min)
                         3/16/2009 14:03 Cooling of bioreactor (Temp setup)
                         3/16/2009 14:03 Purge with 0.5 L/min O2 
                         3/16/2009 14:04 Start Fermentation Software for Fermenter #6 Record 0:00  
                         3/16/2009 14:20 Calibrate dO2 probe with 300 rpm, O2 purge for 30 min                    
                         3/16/2009 14:20 Connect Acid (500 ml) and Base (400 ml), prime pumps
                         3/16/2009 14:20 Start pH control 
                         3/16/2009 14:25 Set dO2
                         3/16/2009 14:30 Completion of bioreactor set up: Ready for inoculation
  -------------------------------------------------------------------------------------------------------
   <Jadwiga>             3/16/2009 14:40 Inoculation: (?? ml) Fermentation 0:00

Day 1 (Mar 17 Tue):

   <Sung-Hye>            3/17/2009 (07:00) F6: Acid (480 ml) Base (320 ml)                                            
   <Sung-Hye>            3/17/2009 (07:30) Increase rpm to 600
                                           Bioreactor picture taken
<Jadwiga> 3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)
<Jadwiga> 3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)
<Jadwiga> 3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)
<Jadwiga> 3/17/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)

Day 2 (Mar 18 Wed):

   <Sung-Hye>            3/18/2009 (07:00) F6: Acid (470 ml) Base (300 ml)
   <Jadwiga>             3/18/2009 (00:00) Sampling Fermentation 00:00
                                            F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)
<Jadwiga> 3/18/2009 (00:00) Sampling Fermentation 00:00 F6: Cell weight ( mg/ml), Activity ( %, x100), Activity ( %, x1000)
<Sung-Hye/Jadwiga> 3/18/2009 (16:00) Fermentation 00:00 Havest and Finish Fermentation F6: Acid (460 ml) Base (300 ml)




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