User:Fermenter User/Notebook/DOE Workshop 20110405

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WORK B1


1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:


When: April 5th - April 14th 2011

Where: LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:

  • Ann Y.K. Wong: Assistant Instructor, Ph.D. previously MacGillivray lab
  • Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
  • Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
  • Swati N. Yewalkar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
  • Shiau-Yun (Mary) Tang (UBC Alumni)


Group A:

(After you log in with your personal password, click the "EDIT" button on the right side of GROUP A and list your name below)

Chris Tam
Louise Creagh

Group B:

(After you log in with your personal password, click the "EDIT" button on the right side of GROUP B and list your name below)

MARY TANG
Swati Yewalkar






Day -1 (April 4th, Mon)

Runs ' ' 1, 2, 3
07:30
08:00
09:00
09:30 SH: Assemble bioreactors
09:50 SH: Autoclve
10:00
10:30
11:10 SH: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation

Day 1 (April 5th, Tue) <-- WORKSHOP STARTS

Runs ' 1, 2, 3 4, 5, 6
pictures
run 1, 2, 3 (left to right)
run 1, 2, 3 (left to right)
08:30 SH: Sampling
09:30 Ann: Prepare high density K91 cells Group A: Assemble bioreactors
09:50 Group A: Autoclave
10:40Presentation Presentation
12:00 Ann: Phage infection Group A: Install bioreactors
13:00 Ann: Spot titer
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # Introduction about our Facility and Services
 # Introduction about bioreactors
 # How to assemble bioreactors


Power Outage started around 8pm and lasted approximately 2.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #4 losted power during fermentation. End point looked ok. 
Run #5 was not affected by power. However, pH control was not done properly. 
Run #6 was not affected by power. End point looked ok.
=> Run #5 will be repeated at last as additional runs.

Day 2 (April 6th, Wed)

Runs 1, 2, 3 4, 5, 6 7, 8, 9
pictures
run 1,2,3 (left to right)
run 1,2,3 (left to right)
run 4,5,6 (left to right)
run 4,5,6 (left to right)
08:00 Ann: Colony counting SH: Sampling
09:00 Ann: Prepare high density K91 cells Group B: Assemble bioreactors
09:50 Group B: Autoclave
10:00PresentationPresentation Presentation
11:00 Ann: Phage infection Group B: Install bioreactors
12:00 Ann: Spot titer
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K91 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # Fermentation Database and Websites
 # How to Install Bioreactors
 # How to Setup Bio-controllers

Another Power Outage started around 6:45pm and lasted approximately 1.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #7 losted power during fermentation. End point looked ok. 
Run #8 was not affected by power. End point looked ok. 
Run #9 was not affected by power. End point looked ok.
=> Run #7 will be repeated at last as additional runs.

Day 3 (April 7th, Thu)

Runs 4, 5, 6 7, 8, 9 10, 11, 12
pictures
run 4,5,6 (left to right)
run 4,5,6 (left to right)
run 7,8,9 (left to right)
run 7,8,9 (left to right)
08:00 Ann: Colony counting SH: Sampling
09:00 Ann: Prepare high density K91 cells
<--didn't grow very well due to power outage
Group A: Assemble bioreactors
09:50 Group A: Autoclave
10:00PresentationPresentation Presentation
11:00 Group A: Install bioreactors
12:00
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K91 cells
17:00 SH: Inoculation


10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 1. Design Experiments

Due to unnotified power outage, two of emergency power lines were introduced into fermentation suite.
One was connected to a computer and one was connected to incubator.
Also, each fermenter is now connected to batteries to provide over 500 min capacity.

Day 4 (April 8th, Fri)

Runs 7, 8, 9 10, 11, 12 11, 13, 14
pictures
colony 7,8,9 (left to right)
colony 7,8,9 (left to right)
run 10,11,12 (left to right)
run 10,11,12 (left to right)
08:00 SH: Sampling
09:00 Ann: Prepare high density K91 cells SH: Assemble bioreactors
09:50 SH: Autoclave
11:00 Ann: Phage Infection SH: Install bioreactors
12:00 Ann: Spot titering
15:30Thaw stock
16:50
17:00Inoculation




NO PRESENTATION

Day 5 (April 9th, Sat)


There is a scheduled power outage on Saturday morning between 8:00 AM and 10:00 AM
=> Power outage began at 8:01 and resumed at 10:00.
No problem occured during this power outage

Runs 6, 8, 9, 10, 12 11, 13, 14 '
pictures
run 10, 12, (left to right)
run 10, 12, (left to right)
run 11,13,14 (left to right)
run 11,13,14 (left to right)
08:00 SH: Colony counting SH: Sampling


NO PRESENTATION

Day 6 (April 10th, Mon)

Runs ' ' 15, 16, 17
07:30
08:00
09:00
09:30 Group B: Assemble bioreactors
09:50 Group B: Autoclve
10:00
10:30
11:10 Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection

Day 7 (April 11th, Tue)

Runs 11, 13, 14 15, 16, 17 18, 19, 20
pictures
run 11, 13, 14 (left to right)
run 11, 13, 14 (left to right)
run 15,16,17 (left to right)
run 15,16,17 (left to right)
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 SH: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics

Day 8 (April 12th, Wed)

Runs 15, 16, 17 18, 19, 20 4, 5, 7
pictures
run 15, 16, 17 (left to right)
run 15, 16, 17 (left to right)
run 18,19,20 (left to right)
run 18,19,20 (left to right)
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check SH: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis: Model Graph and Step 4. Optimization

Day 9 (April 13th, Thu)

Runs 18, 19, 20 4, 5, 7 '
pictures
run 18, 19, 20 (left to right)
run 18, 19, 20 (left to right)
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check SH: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00
10:00 - 11:00 Presentation (Rm 4.303)
 # Case Studies: Publication

Day 10 (April 14th, Fri)

Runs 4, 5, 7 ' '
07:30
08:00
09:00 Group B: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)

Helful links

  1. http://meta.wikimedia.org/wiki/Table
  2. http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!






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