User:Fermenter User/Notebook/DUON A1

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

DUON A1 (F3)


Day -1 (Feb 5 Thu):

   
    Sung-Hye: CHBE Class Auditing 9:30 - 11:00
   
   <Jean-Francois>      2/5/2009 (12:10) Bring media to fermenter room
<Sung-Hye> 2/5/2009 (12:20) Assemble bioreactor 2/5/2009 (12:20) Add 2 ml of Antifoam. 2/5/2009 (13:30) Start Sterilization (Liq 45 min)


Day 0 (Feb 6 Fri):

                        2/6/2009 (7:00) Cooling of bioreactor (Temp setup) 2 L/min air, 800 rpm
                        2/6/2009 (8:45) Calibrate dO2 probe with 250 rpm, O2 purge for 30 min
                        2/6/2009 (8:45) Set dO2
                        2/6/2009 (8:45) Start OD module software
                        2/6/2009 (8:50) Start Fermentation Software for Fermenter #3
                        2/6/2009 (9:00) Completion of bioreactor set up: Ready for inoculation

-----------------------------------------------------------------------------------------------------

<JF and SH> 2/6/2009 (11:10) Add Amp (5 ml) 2/6/2009 (11:10) Inoculation: (50 ml) Fermentation 0:00 <Jean-Francois> 2/6/2009 (12:43) Sampling Fermentation 1:33 AU (0.05) OD (0.27) <Jean-Francois> 2/6/2009 (13:00) Sampling Fermentation 2:50 AU (0.08) OD (0.50) <JF and SH> 2/6/2009 (13:08) Fermentation 2:58 Induction 0:00 Add Inducer 20% Arabinose, 50 ml AU (0.10) <Jean-Francois> 2/6/2009 (13:09) Sampling Fermentation 2:59 Induction 0:01 AU (0.10) OD (0.55) <Jean-Francois> 2/6/2009 (13:28) Sampling Fermentation 3:18 Induction 0:20 AU (0.14) OD (0.75) <Jean-Francois> 2/6/2009 (14:18) Sampling Fermentation 4:08 Induction 1:10 AU (0.39) OD (2.2) <Jean-Francois> 2/6/2009 (16:00) Sampling Fermentation 5:50 Induction 2:48 AU (0.63) OD (3.9) <Jean-Francois> 2/6/2009 (19:15) Sampling Fermentation 9:05 Induction 6:03 AU (0.79) OD (4.4)

Day 1 (Feb 7 Sat):

     <Sung-Hye>           2/7/2009 (11:00) Fermentation 24:50 Induction 21:48
                                        Harvest




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