User:Fermenter User/Notebook/MACG G1

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Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs
Pink: MeOH Induction hrs

MACG G1 (F1)

Contents

Day -4 (Jul 17 Fri):

<Ganna>              6/17/2009 (00:00)     Prepare and autoclave additives:

Day -1 (Jul 20 Mon):

<Ganna>              6/20/2009 (11:45)     Bring YP (1-L) to fermenter room
<Sung-Hye>           6/20/2009 (11:45)     Calibratiron of pH sensor
                                           Add 1 ml of Antifoam
                     6/20/2009 (12:40)     Autoclave start                          
                     6/20/2009 (14:10)     Start Fermenter #1 software
                     6/20/2009 (14:10)     Set rpm at 900 
                     6/20/2009 (14:10)     Start temp control  


<Ganna>              6/20/2009 (10:30)     Take a colony from plate in 600 ml of BMGY(4 500ml flasks).
   
                                           Allow it to gorw at 30°C.

Day 0 (Jun 21 Tue): == MeOH Induction Day 1

<Sung-Hye>           6/20/2009 (09:30 -10:30) Feed additives using a pump
                                           Increase rpm to 1250 (Max)
                     6/20/2009 (10:40)     Calibrate dO2 sensor
<Ganna>              6/21/2009 (14:00)     Suspend down cells and resuspend in 15 ml of BMMY.
                                           Set up a 0.5 L culture in buffled flask.  
          
<Ganna/SungHye>      6/21/2009 (14:30)     Fermentation 00:00
                                           Inoculate into fermenter

Day 1 (Jun 22 Wed): == MeOH Induction Day 2

<Ganna/SungHye>      6/22/2009 (00:00)     Fermentation 00:00
                                           Add 0.5% MeOH


Day 2 (Jun 23 Thu): == MeOH Induction Day 3

<Ganna/SungHye>      6/23/2009 (00:00)     Fermentation 00:00
                                           Add 0.5% MeOH

Day 3 (Jun 24 Fri): == MeOH Induction Day 4

<Ganna/SungHye>      6/24/2009 (00:00)     Fermentation 00:00
                                           Add 0.5% MeOH
<Sung-Hye>           6/24/2009 (00:00)     Fermentation 00:00
                                           Harvest

          


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