User:Fermenter User/Notebook/STRY C2T

From OpenWetWare

Jump to: navigation, search
Customize your entry pages


Background color codes:
Pastel Green: Major change of setup this time
yellow: Sung-Hye's correction or question to user

Letter color codes:
Black: Basic comment
Red: Emergency Information (Power shutdown, building access, Manager's absence etc)
Blue: Fermentation Setup information
Green: Discussion
Orange: Fermentation hrs

STRY C2T


Day -1 (Apr 14 Tue):

   <Frank>               4/14/2009 11:20 Bring powder to fermenter room
   -------------------- Set up of F6 ------------------------------------------------
   <Sung-Hye>            4/14/2009 11:20 Calibrate pH sensor
                         4/14/2009 11:20 Make 5-L media
                         4/14/2009 11:20 Add 1 ml of antifoam                            
                         4/14/2009 11:26 Autoclave
                         4/14/2009 12:40 Cool down bioreactor
                                        800 rpm, 1-L/min air
                         4/14/2009 12:42 Start software                        
   -------------------- Set up of F4 ------------------------------------------------
   <Sung-Hye>            4/14/2009 12:00 Calibrate pH sensor
                         4/14/2009 12:20 Make 50-L media
                         4/14/2009 12:20 Add 10 ml of antifoam  
                         4/14/2009 12:30 Start software                        
                         4/14/2009 12:40 Start Sterilization routine
                         4/14/2009 14:00 Cool down bioreactor
                                        800 rpm, 50-L/min air

Day 0 (Apr 15 Wed):

   ---------------------- Set up of F6 -----------------------------------------------
                         4/15/2009 07:00 Connect Base (400 ml)
                         4/15/2009 07:00 Set pH
                         4/15/2009 07:00 Calibrate dO2 probe 
                         4/15/2009 07:00 Set dO2
   ---------------------- Set up of F4 -----------------------------------------------
                         4/15/2009 07:00 Connect Base (875 ml)
                         4/15/2009 08:00 Set pH
                         4/15/2009 08:00 Calibrate dO2 probe 
                         4/15/2009 08:00 Set dO2 
   <Frank>               4/15/2009 11:00  Add antibiotics
   <Frank>               4/15/2009 11:00  Fermentation 0:00
                                         Inoculation
                                         F4: 1000 ml
                                         F6: 10 ml
   <Frank>               4/15/2009 14:30  Fermentation 03:30
                                         Sampling (F4): OD (0.9)
                                         Sampling (F6): OD (0.15)
<Sung-Hye> 4/15/2009 15:30 Fermentation 04:30 Change temperature <Sung-Hye> 4/15/2009 16:30 Fermentation 05:30 Fermentation 00:00 Add inducer

Day 1 (Apr 16 Thu):

   <Sung-Hye>            4/16/2009 07:00 Fermentation 20:00 Fermentation 14:30
                           F4: Base (600 ml)
                           F6: Base (390 ml)
   <Frank>               4/16/2009 10:30 Fermentation 23:30 Fermentation 18:00
                           F4: OD 2.1
                           F6: OD 1.4
   <Frank>               4/16/2009 15:00 Fermentation 28:00 Fermentation 22:30
                           F4: OD 2.5
                           F6: OD 1.5

Day 2 (Apr 17 Fri):

   <Sung-Hye>            4/17/2009 07:00 Fermentation 20:00 Fermentation 14:30
                           F4: Base (580 ml)
                           F6: Base (390 ml)
   <Frank>               4/17/2009 10:30 Fermentation 23:30 Fermentation 18:00
                           F4: OD 2.1
                           F6: OD 1.4
   <Sung-Hye>            4/17/2009 13:30  Havest and Finish Fermentation



Recent changes



Personal tools