User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/21

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Objectives

  1. Run fluorescence data for lysozyme at pH=4
  2. Create stock solutions for use on 09.27.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 5 minutes for 3 hours

Solutions for 09/27/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. concentration = 225 μM
    2. Gold Stock Solution
      1. concentration = 3.73 mM
    3. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 6
      1. 223 μL AuCl3
      2. 3 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2607 μL Water
  2. Fluorescence Summary Graphs

Figure 1 Maximum emission data in a solution of pH 6


Figure 2 Integrated fluorescence data in a solution of pH 6


Figure 3 Full emission spectra for important time points: t=0, 6, 39, 96, and 180 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=6 min, this maximum has shifted up and to the right.
  3. Until t=39 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=39 min, the maximum begins increasing again.
  5. At t=96 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=180 shows the final full spectra obtained.

UV-vis solutions for 09.27.2016
Image:092716 UVvis sol Lys fruc75.png
Table 1 UV-vis solutions made for [Fructose]=.75mM

Image:092716 UVvis Lys fruc125.png
Table 2 UV-vis solutions made for [Fructose]=1.25mM

Notes

Fluorescence data taken every 3 minutes instead of every 5 minutes


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