User:Helen L. Slucher/Notebook/CHEM-572/2014/02/25

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Objective

  • Make 300mL GNPs
  • Make more dopamine
  • Make buffer (de-gas tomorrow)

Dopamine

  • Make tubes with three dopamine solutions
  1. Gray (bad)
  2. Gray (bad)
  3. Purple (good)
  • Add HRP or hemoglobin to each tube

Buffer

  1. Tris
  2. Tris and NaDDT

Notes

  • Dopamine tubes with proteins will be left in refrigerator to react overnight
  • De-gas buffer tomorrow
  • Add NaDDT to buffer tomorrow
  • Buffers had a pH of 7.5


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