User:Helen L. Slucher/Notebook/CHEM-572/2014/04/01

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Goals

  1. Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
  2. Spin down gamma method HRP pellet
  3. Remake solutions so they are more concentrated

Notes: Kinetics

  • Reduction of heme group
  • OPD, look for diamino...change of concentration over time, rates
  • Change in absorbance = change in concentration
  • Find molar absorptivity
  • Deoxygen/oxygen bound
  • Take off clear super, spin down until it doesn't show GNP activity



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