User:Helen L. Slucher/Notebook/CHEM 571/2013/10/15

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Objective Make HRP-AuNPs for enzymatic studies Procedure HRP-AuNP Solutions 30:1 Au:HRP 5.10 mL HRP and 850.34 μL Au 60:1 Au:HRP 2.56 mL HRP and 850.34 μL Au 90:1 Au:HRP 1.71 mL HRP and 850.34 μL Au 120:1 Au:HRP 1.28 mL HRP and 850.34 μL Au The final volume was 10 mL, diluted with water Synthesis of HRP-AuNPs Each solution was placed in a test tube The test tubes were placed in the oven at 80°C for approximately 3 hours Notes Stock solution of Au: 2.94 mM. Stock solution of HRP: 16.27 μM.