User:Howard Boland/Notebook/Art from Synthetic Biology/2010/11/24
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Gel of Digestion
I decided to also the my ligation part to see how or which one that works since my 2.3kb product broke down into 1600bp and 700bp.
I set up three tubes
I left the samples in room temperature for 2 hours
Kanamycin I prepared 7 plates of Agar with Kanamycin using 200ml LB-Agar and 1ml Kanamycin.
Ampicillin / Tetracycline I prepared 7 plates of Agar with AT/AT (Ampicillin/Tetracycline) using 200ml LB-Agar, 200µl Ampicillin and 200µl Tetracycline.
I transformed the 3 ligations using heat-shock and also I did a fourth transformation using the part BBa_J45200 taken from F5 of the 2nd plate of the 2010 Spring Distribution Plate.
I incubated the plates overnight at 37ºC
Setting-up disk test plate for H2O2
I prepared a new serial dilution of 1/10 and 1/2 H2O of 30% H2O2. (1/1000, 1/10000, 1/20000, 1/40000, 1/80000). A plate was prepared by streaking out glycerol stock over the entire area using a swab. The disk were then placed in a clockwise order from beneath and the plate was left in an incubator to grow overnight.