User:Howard Boland/Notebook/Art from Synthetic Biology/2011/02/10

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Check Transformations

All plates transformed well

Overnight Growth pSB1C3

2 colonies where produced

Overnight growth backbones

  • pSB1A3
  • pSB1K3
  • pSB1T3

Digestion backbones

All plasmids where digested (30µl each) using EcoRI and PstI

  • pSB1A3
  • pSB1K3
  • pSB1T3

Gel backbones

1% Agarose Gel, 10-FEBRURARY-2011-17:44 GMT

  1. Lane 1: 10µL 1kbp NEB Quick Ladder
  2. Lane 2: 50µl pSB13A - EcoRI / PstI
  3. Lane 3: 5µl pSB13A - plasmid
  4. Lane 4: 50µl pSB13K - EcoRI / PstI
  5. Lane 5: 5µl pSB13K - plasmid
  6. Lane 6: 50µl pSB13T - EcoRI / PstI
  7. Lane 7: 5µl pSB13T - plasmid
  8. Lane 8: BLANK

Image:10-feb-2011-backbone-digests.jpg

Ligation

  1. 7µl BBa_katE E|S
  2. 7µl BBa_I13504 X|P
  3. 3µl pSB13K E|P



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