Check Transformation
Few colonies (3-8)
- 1>6I, XL-1 BLUE, ok
- 1>12O, XL-1 BLUE, ok
- 1>22I, XL-1 BLUE-a, ok
Check Overnight Growth
- 1>6I, XL-1 BLUE, ok > 1.2 O.D. -> Move 1ml over to new bottle and grow to 0.5 O.D.
- 1>6I, XL-1 BLUE, ok > 1 O.D. -> Move 1ml over to new bottle and grow to 0.5 O.D.
Miniprep
- 1>6I, 1.5ml 0.5 O.D. -> elute in 50µl H20
- 1>6I, 1.5ml 0.5 O.D. -> elute in 50µl H20
- 1>6I, 5ml 0.5 O.D. -> elute in 50µl H20
- 1>6I, 5ml 0.5 O.D. -> elute in 50µl H20
- 1>6I, 6ml 1.2 O.D. -> elute in 50µl H20
- 1>6I, 6ml 1 O.D. -> elute in 50µl H20
Gel Plasmids
- 5µl 1kb ladder
- 5µl 1>6I, 1.5ml 0.5 O.D.
- 5µl 1>6I, 1.5ml 0.5 O.D.
- 5µl 1>6I, 5ml 0.5 O.D.
- 5µl 1>6I, 5ml 0.5 O.D.
- 5µl 1>6I, 6ml 1.2 O.D.
- 5µl 1>6I, 6ml 1 O.D.
- 30µ1>22I from Friday
Overnight Growth
3 bottles of 10ml culture inoculated from todays transformation and 10µl Ampicillin per bottle
- 1>12O, E0840
- 1>22I, I13504
- 1>6I, E0040
Transformation
Setup 5 transformations
- 1>12O, XL-1 BLUE (A.A.), ok
- 1>12O, XL-1 BLUE, ok
- 1>12O, DH5-a, blank
- 1>22I, DH5-a, blank
- 1>22I, XL-1 BLUE, blank
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Project name
Main project page
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