User:Howard Boland/Notebook/Art from Synthetic Biology/2011/03/07

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Check Transformation

Few colonies (3-8)

  1. 1>6I, XL-1 BLUE, ok
  2. 1>12O, XL-1 BLUE, ok
  3. 1>22I, XL-1 BLUE-a, ok

Check Overnight Growth

  1. 1>6I, XL-1 BLUE, ok > 1.2 O.D. -> Move 1ml over to new bottle and grow to 0.5 O.D.
  2. 1>6I, XL-1 BLUE, ok > 1 O.D. -> Move 1ml over to new bottle and grow to 0.5 O.D.

Miniprep

  1. 1>6I, 1.5ml 0.5 O.D. -> elute in 50µl H20
  2. 1>6I, 1.5ml 0.5 O.D. -> elute in 50µl H20
  3. 1>6I, 5ml 0.5 O.D. -> elute in 50µl H20
  4. 1>6I, 5ml 0.5 O.D. -> elute in 50µl H20
  5. 1>6I, 6ml 1.2 O.D. -> elute in 50µl H20
  6. 1>6I, 6ml 1 O.D. -> elute in 50µl H20

Gel Plasmids

  1. 5µl 1kb ladder
  2. 5µl 1>6I, 1.5ml 0.5 O.D.
  3. 5µl 1>6I, 1.5ml 0.5 O.D.
  4. 5µl 1>6I, 5ml 0.5 O.D.
  5. 5µl 1>6I, 5ml 0.5 O.D.
  6. 5µl 1>6I, 6ml 1.2 O.D.
  7. 5µl 1>6I, 6ml 1 O.D.
  8. 30µ1>22I from Friday

Overnight Growth

3 bottles of 10ml culture inoculated from todays transformation and 10µl Ampicillin per bottle

  1. 1>12O, E0840
  2. 1>22I, I13504
  3. 1>6I, E0040

Transformation

Setup 5 transformations

  1. 1>12O, XL-1 BLUE (A.A.), ok
  2. 1>12O, XL-1 BLUE, ok
  3. 1>12O, DH5-a, blank
  4. 1>22I, DH5-a, blank
  5. 1>22I, XL-1 BLUE, blank



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