User:Howard Boland/Notebook/Art from Synthetic Biology/2011/03/08

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Check Transformation

  1. 1>12O, XL-1 BLUE, ok
  2. 1>12O, XL-1 BLUE, ok
  3. 1>12O, DH5-a, blank
  4. 1>22I, DH5-a, blank
  5. 1>22I, XL-1 BLUE, blank

Overnight Growth

  1. 1>12O, E0840, Ok (1.5OD) -> Stock + Moved 1ml into fresh broth and continue growing
  2. 1>22I, I13504, Ok (0.9OD) -> Stock + Moved 1ml into fresh broth and continue growing
  3. 1>6I, E0040, appears dark (possible smudging of from plastic in bottle) -> Stock + Moved 1ml into fresh broth and continue growing

Miniprep

  1. 5ml 1>12O, E0840 -> Elute in 35µl H20
  2. 5ml 1>22I, I13504 -> Elute in 35µl H20

Gel D.D. R0040

  1. 10µl 1kb Ladder
  2. BLANK
  3. 30µl R0040 E/S (H.B.)
  4. BLANK
  5. 30µl R0040 E/S (A.A.)
  6. BLANK
  7. 5µl R0040 E/S (H.B.)
  8. 5µl R0040 E/S (A.A.)

Gel Plasmids

  1. 5µl 1kb Ladder
  2. BLANK
  3. BLANK
  4. 5µl 12O
  5. BLANK
  6. 5µl 22I
  7. BLANK
  8. 10µl R0040 (0.5OD>5ml>A.A)


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