User:Jacob Esenther/Notebook/581/2014/10/01

Biomaterials Design Lab

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Objective Today, we are looking at the diffusion of Malachite Green across a film. Description Diffusion Our group chose to use a PVA/clay film Michael's group is using a PVA film. Their protocol may be viewed here. the films were set between the two chambers Added liquid to each chamber One chamber got DI water The other chamber got 20ppm Malachite green Stock solution made by Mary, see here. At the 5 minute mark, 200uL was taken from each chamber and placed in an eppy tube Measure the UV-Vis spectrum for both samples using a small volume cuvette. Return each to the chamber it originally came from Take measurements at the following times 15 minutes 30 minutes 1 hour 2 hours right before you leave lab Data Mass of Film Piece - 0.8846g Width of Film Piece - 0.51mm Diameter of Diffusion Tube - 14.41mm Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.