User:Jacob Esenther/Notebook/Chem 571/2014/09/09

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Objective

  1. Take the UV-VIS spectra of amino acid solutions that were produced on September 3.
  2. Fluorescence of Amino Acids
  3. Repeat kinetics experiment originally preformed on August 27


Description

  1. Take the UV-VIS spectra of amino acid solutions that were produced on September 3.
    • 2-3 mL samples of each of the amino acid stock solutions were loaded into polystyrene cuvettes, one at a time, and the data gathered was used to create the graphs below.
  2. Fluorescence of Amino Acids
    1. A fluorescence spectrum of 81.2nM L-Tryptophan was run at a wavelength of 275nm.
      • the concentration used resulted from a 2500:1 dilution of a 203μM solution of L-Tryptophan
      • L-Tryptophan was the only amino acid that produced significant results with UV-VIS, hence the only one that required a fluorescence
  3. Repeated kinetics experiment
    1. 0.5mL HAuCl4 was put in 5 test tubes
    2. enough lysozyme was added to make a [Au]:[Lysozyme]=30 solution.
      • The chart in the notes segment illustrates concentrations necessary.
    3. Test tubes were covered with foil and placed in an 80°C hot water bath.
    4. Measurements were taken every 20 minutes on the UV-VIS
      • Measurements were taken from 800-260nm


Data

Amino Acid Absorbance Spectrum

Absorbance of Glycine Solution
Absorbance of Glycine Solution
Absorbance of L-Cysteine Solution
Absorbance of L-Cysteine Solution
Absorbance of L-Glutamine
Absorbance of L-Glutamine
Absorbance of L-Lysine Solution
Absorbance of L-Lysine Solution
Absorbance of L-Tryptophan Solution
Absorbance of L-Tryptophan Solution
Fluorescence Spectrum of L-Tryptophan
Fluorescence Spectrum of L-Tryptophan

Kinetics spectrum

Kinetics of AuNP Formation with Lysozyme
Kinetics of AuNP Formation with Lysozyme

Notes

This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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