User:James C. Schwabacher/Notebook/CHEM-571/2013/09/10
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Utilize the Bradford Assay to make calibration curves for Horseradish Peroxidase (HRP).
- Note: This is where coordination is a good thing. Take 1 spectrum at a time. Let other people go.
Note - Solutions from today containing the stain will go into a waste container in the hood.
We are also going to make Atomic Absorption standards for tomorrow!
Using the gold AA/ICPMS standard solution make 5 new solutions (Note: Use water - NOT BUFFER - to make these solutions)
Procedure Adapted from Prof. Hartings
All data collected above was collected using the shortened path length of .412 cm. The data collected from trials 1 and 2 was not accurate, so a trial 3 was collected. Trial 3 was also not accurate, as shown through the calibration curves. When corrected for a set y-intercept of 0, the r-squared values become negative for both calibrations. Also, the procedure was further amended for trial 3, during which the dilutions were made using 10 ml, and then transferred to the cuvette,in order to allow for larger, more accurate measurements of stock solution. We will attempt a fourth data collection tomorrow.
The gold standard solution was made using a small sample of the stock solution diluted in a 10ml volumetric flask with distilled water.