User:James C. Schwabacher/Notebook/CHEM-571/2013/10/15

Biomaterials Design Lab

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Objective To make HRP-AuNPs for enzymatic studies. Procedure Creating the Solutions of Gold and HRP For 30:1 Au:HRP solution, 5.10 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water. For 60:1 Au:HRP solution, 2.56 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water. For 90:1 Au:HRP solution, 1.71 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water. For 120:1 Au:HRP solution, 1.28 mL HRP stock and 850.34 μL of gold stock were added to a 10 mL volumetric flask. The solution was diluted up to 10 mL with deionized water. Synthesis of HRP-AuNPs The 10 mL solutions of gold and HRP were placed into four different test tubes. The test tubes were placed in the oven at 80°C for approximately 3 hours. From 12:40pm to 3:50pm. Notes The stock solution of gold was 2.94 mM. The stock solution of HRP was 16.27 μM.