- Make a column for solid-phase extraction and concentration of BPA samples.
- Packing the Column
- To pack the column we got a syringe with a stop cock at the bottom.
- We packed the column with glass wool and washed it with methanol to help pack.
- Next, a layer of sand was put on top
- A solution of 9.9999g of Silica in methanol was poured into the column.
- After draining the column was washed with DI water until the column ran clear.
- Then 10mL of BPA and let it run into the layer of silica.
- then 10mL of water, then 30mL of methanol were run into the column
- These were divided into 8 5mL fractions.
TLC plates did not show any evidence of BPA--it is believed that the concentration (10 ppm) is too low to effectively use this method.
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