User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/24

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Objective

  • Complete the QD synthesis begun yesterday
  • Continue precipitation of JCS 1 and JCS 2
  • Begin precipitation of JCS 3

Protocol

  1. Create 20 mM sodium sulfide solution
    1. .024018 g of Sodium Sulfide (MW=240.18) needed for 5 mL
    2. Used .1373 g of Sodium Sulfide used (in 5 mL) because the sample is contaminated with excess water
  2. Add 4 mL of 20 mM sodium sulfide solution to reaction
    1. Allowed the mixture to stir vigorously for 15 minutes
    2. Poured contents (minus the stir bar) into a darkened falcon tube and filled with 200 proof ethanol to the 50 mL mark. The falcon tube was left in the freezer to begin precipitation.
    3. total synthesis time: 13 hrs (including 15 minutes for Sodium Sulfide addition)
  3. Removed 1 mL aliquot from JCS 3 (before adding ethanol) for future testing with UVVIS and Fluorescence

Precipitation of JCS 1 and 2

  1. Falcon tubes spun at 4000 RPM for 15 minutes
  2. Solvent was decanted and stored while the dark precipitate was re-suspended in ~15 mL of distilled water.
  3. Solutions were stored in the dark at room temp for future analysis with UVVIS and fluorescence.

Notes

  • JCS 1 remained mostly clear with minimal dark precipitate, as it was before it was spun.
  • JCS 2 remained darkened brown. After spinning some precipitate was visible
  • Eleni and I feel that the next step is to try centrifugation at very high speeds, which was necessary with some of the Mg:ZnS quantum dots we created last semester.


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