User:James C. Schwabacher/Notebook/Protein-Templated Quantum Dots/2014/09/09

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Objective

  • Synthesize HgS-BSA QDs at room temperature|JCS 1

Protocol

  1. Making Stock Solutions
    1. 4.0222 g of NaOH in 100 mL ~1M NaOH
    2. .0736 g HgNO3 in 50 mL of ~9 pH Distilled Water
    3. .0728 g HgNO3 in 50 mL of HPLC grade water
  2. Synthesis
    1. Added 5 mL of 15mg/mL BSA from Eleni's stock solution to 5 mL of the HgNO3 in HPLC water
    2. stirred vigorously while adding 2 drops of 1 M NaOH to reach pH ~9
    3. capped to sit and react for ~12 hours

Notes

  • Both HG solutions did not dissolve readily. The solutions were vortexed and sonicated. The sample was vortexed again before removing 5 mL for the synthesis.
  • The pH test trip was slightly darker than the coding for a pH 9. I have some concern that the pH of this first batch might be too high.


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