User:James Chappell/ CO2 bacteria

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Home The Project B.subtilis Chassis Wet Lab Dry Lab Notebook


Wet Lab

Cloning Strategy


In order to expand the use of B.subtilis as a potential chassis we investigated the different protocols used for the transformation of B.subtilis. B.subtilis is used as the model for gram positive bacterium and have been extensively studied. A variety of protocols have been developed for the transformation of B.subtilis. We wished to investigate two different protocols, one relying upon natural competence of stressed B.subtilis and the using electroporation.


Cloning Strategy


In order to expand the use of B.subtilis as a potential chassis we investigated the different protocols used for the transformation of B.subtilis. B.subtilis is used as the model for gram positive bacterium and have been extensively studied. A variety of protocols have been developed for the transformation of B.subtilis. We wished to investigate two different protocols, one relying upon natural competence of stressed B.subtilis and the using electroporation.


Calibration Curves
Testing

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