User:Jamie Nunziata/Notebook/Protease Research/2015/10/28

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Objective

The objective of today's lab was to use the Ocean Optic spectrometer to measure the absorbance and determine the rate of the degradation of AuNP fibers from 100nM a-chymotrypsin.


Procedure

4 fiber samples were spun down at 300rpm for 10 minutes and was carefully brought into solution from 1mL of Tris buffer, keeping the fibers as intact as possible. 1995.26µL of Tris buffer and all 4 fiber samples were added to the Ocean Optic cuvette. Immediately after the second save file was added, 4.74µL of 42.188µM a-chymotrypsin was added to the cuvette, making the final molarity 100nM. Absorbance was collected for 24 hours at various parameters: every 30 seconds for about 1 hour, every 2.5 minutes for about another hour, and every 20 minutes for the remainder of the readings.


Data

Image:JMN_10_28_2015_OO_CorrectedAbsorbance.jpg



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