User:Jarle Pahr/Library preparation

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Notes on preparation of nucleic acid libraries:


Kit Hands-on time Total time Supplier Platforms DNA requirement Link
TruSeq
Nextera
Moleculo
NEBNext ? NEB
Ion AmpliSeq



Contents

Illumina

Truseq: Illumina TruSeq DNA sample preparation guide: http://nextgen.mgh.harvard.edu/attachments/TruSeq_DNA_SamplePrep_Guide_15005180_A.pdf


Nextera:


Nextera DNA sample preparation kits: http://www.illumina.com/products/nextera_dna_sample_prep_kit.ilmn

Nextera sample preparation guide: http://supportres.illumina.com/documents/myillumina/2a3297c5-8a34-4fc5-a148-3e16666fd65e/nextera_dna_sample_prep_guide_15027987_b.pdf

Nextera DNA sample preparation best practices video: http://support.illumina.com/sequencing/sequencing_kits/nextera_dna_kit/training.ilmn

Nextera DNA sample preparation course video: http://support.illumina.com/sequencing/sequencing_kits/nextera_dna_kit/training.ilmn


Moleculo:

PacBio

PacBio SMRT sample prep: http://www.smrtcommunity.com/SampleNet/Sample-Prep


454

http://my454.com/downloads/my454/applications-info/454SequencingSystem_GuidelinesforAmpliconExperimentalDesign_July2011.pdf


Ion torrent

http://integenx.com/wp-content/uploads/2012/07/PrepX-PGM_flyer-IT.pdf

NEBNext: https://www.neb.com/products/e6285-nebnext-fast-dna-fragmentation-and-library-prep-set-for-ion-torrent

http://www.invitrogen.com/1/3/library-prep-for-sequencing-ion


Ion AmpliSeq library kit 2.0: https://products.invitrogen.com/ivgn/product/4475345

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