User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2013/10/15

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Entry title Insert content here... Objective Today we are going to be making HRP-AuNPs for enzymatic studies. Description Make 4 solutions of HRP-AuNPs Au:HRP ratio - 30:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL) Au:HRP ratio - 60:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL) Au:HRP ratio - 90:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL) Au:HRP ratio - 120:1 HRP-Au (final concentration of Au - 0.25mM; total volume - 10mL) After taking the test tubes from the oven, it was observed that the solutions had the purple color needed. Data Stock solutions Gold 25.0mg HAuCl4·H2O in 25.0mL water --> 2.94mM HRP 17.9mg HRP in 25.0mL water --> 16.27μM