User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/02/26

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Objective

Today we'll be recording the results of 30:1 lysozyme AuNPs with variables added before heating at 37 degrees Celsius overnight. Then we will take UV-Vis of the samples with salts added after heating overnight, and we will synthesize 30:1 lysozyme AUNPs, and 60:1,70:1,80:1,90:1,110:1 BSA-AuNPs with MgCl,sub.2 for 4 hours at 80 degress.

Procedure

Synthesizing Lysozyme-AuNP and BSA-AuNP

  • The following figure details how the stock solutions were made as well as the BSA-AuNPs and lysozyme-AuNPs.
  • One tube of each ratio was made for BSA-AuNPs.
  • For lysozyme-AuNPs, 25 tubes were made.
  • The samples were heated in the oven at 80°C for 4 hours.

Figures

UV-Vis of Heated Samples and Variables (36°C)

Image:Screen_shot_2014-02-26_at_5.18.42_PM.png Image:Screen_shot_2014-02-26_at_5.18.29_PM.png Image:Screen_shot_2014-02-26_at_5.18.18_PM.png Image:Screen_shot_2014-02-26_at_5.17.57_PM.png Image:Screen_shot_2014-02-26_at_5.17.46_PM.png Image:Screen_shot_2014-02-26_at_5.17.36_PM.png Image:Screen_shot_2014-02-26_at_5.17.23_PM.png

Notes

These results sum up the detail of the samples as seen below, but being at 37 degrees before observations. After observations, the samples were placed in the fridge.

These results detail the samples from last Wednesday with the addition of the variables to 1 mL aliquots of 30:1 ratio with salts added after. The samples were at room temperature (37°C) when observations were taken.


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